|Application ||WB, IF|
|Calculated MW||38571 Da|
|Positive Control||Western blot: HL-60 cell lysate, IF: Sy5Y cells|
|Application & Usage||Western blot: ~1:1000, IF: ~1:10–1:50.|
|Other Names||POU5F1; OCT3; OCT4; OTF3; POU domain, class 5, transcription factor 1; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3|
|Formulation||Supplied in PBS with 0.09% (W/V) sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||OCT4 (OCT3) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||OCT3, OCT4, OTF3|
|Function||Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.|
|Cellular Location||Cytoplasm. Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern|
|Tissue Location||Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.|
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Provided below are standard protocols that you may find useful for product applications.
Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells. A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation. Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker. Oct-4 exists as two splice variants, Oct-4A and Oct-4B. Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells.
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