|Application ||WB, IF, IP|
|Reactivity||Human, Mouse, Rat, Bovine|
|Positive Control||Western blot: 293 cell lysate, FACS: 293 cells|
|Application & Usage||Western blotting (1-2 µg/ml), immunoprecipitation (20-40 µg/ml), and immunocytochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects Myc-Tag proteins overexpressed in cells. It does not detect endogenous levels of the transcription factor c-Myc.|
|Other Names||MYC; BHLHE39; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; Transcription factor p64|
|Formulation||Supplied in in PBS with 0.09% (W/V) sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||C-Myc Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Epitope tags are usually for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian systems.
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