Vimentin Antibody (CT)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, IF, FC |
---|---|
Primary Accession | P08670 |
Reactivity | Human, Mouse, Rat, Hamster, Monkey, Pig, Chicken, Xenopus, Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 53652 Da |
Gene ID | 7431 |
---|---|
Positive Control | Western blot: NCI-H460 cell lysate, IHC: human colon tissue, FACS: HeLa Cells, IF: SY5Y cells. |
Application & Usage | Western blot: ~1:1000, IHC: 1:10 – 1:50, FACS: 1:10 – 1:50, IF: 1:100. |
Other Names | Vimentin, VIM |
Target/Specificity | Vimentin |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | In PBS with 0.09% (W/V) sodium azide. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Vimentin Antibody (CT) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | VIM |
---|---|
Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. |
Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}. Cell membrane {ECO:0000250|UniProtKB:P20152} |
Tissue Location | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, α-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently co-expressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.
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