|Application ||WB, IHC, IF, FC|
|Reactivity||Human, Mouse, Rat, Hamster, Monkey, Pig, Chicken, Xenopus, Bovine|
|Calculated MW||53652 Da|
|Positive Control||Western blot: NCI-H460 cell lysate, IHC: human colon tissue, FACS: HeLa Cells, IF: SY5Y cells.|
|Application & Usage||Western blot: ~1:1000, IHC: 1:10 – 1:50, FACS: 1:10 – 1:50, IF: 1:100.|
|Other Names||Vimentin, VIM|
|Formulation||In PBS with 0.09% (W/V) sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Vimentin Antibody (CT) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.|
|Tissue Location||Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines|
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Provided below are standard protocols that you may find useful for product applications.
Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, α-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently co-expressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.
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