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Vimentin Antibody (CT)

Rabbit Polyclonal Antibody

  • IF - Vimentin Antibody (CT) ABV11317
    Fluorescent confocal image of SY5Y cells stained with Vimentin (C-term) antibody. Cells were incubated with Vimentin (C-term) antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).
  • IHC - Vimentin Antibody (CT) ABV11317
    Vimentin Antibody (CT) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08670
Reactivity Human, Mouse, Rat, Hamster, Monkey, Pig, Chicken, Xenopus, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 53652 Da
Additional Information
Gene ID 7431
Positive Control Western blot: NCI-H460 cell lysate, IHC: human colon tissue, FACS: HeLa Cells, IF: SY5Y cells.
Application & Usage Western blot: ~1:1000, IHC: 1:10 – 1:50, FACS: 1:10 – 1:50, IF: 1:100.
Other Names Vimentin, VIM
Target/Specificity Vimentin
Antibody Form Liquid
Appearance Colorless liquid
Formulation In PBS with 0.09% (W/V) sodium azide.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsVimentin Antibody (CT) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines
Citations (0)

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Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, α-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently co-expressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.

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$ 275.00
Cat# ABV11317
(40 western blots)
Availability: 5-7days
Bulk Size
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