|Application ||WB, IHC, FC|
|Positive Control||Western blot: CEM cell lysate, IHC: human tonsil tissue, FACS: NCI-H460 cells.|
|Application & Usage||Western blot: ~1:1000, IHC: 1:10 – 1:50, FACS: 1:10 – 1:50.|
|Other Names||RUNX2; AML3; CBFA1; OSF2; PEBP2A; Runt-related transcription factor 2; Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; Polyomavirus enhancer-binding protein 2 alpha A subunit; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit|
|Formulation||In PBS with 0.09% (W/V) sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Runx2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The mammalian Runt-related transcription factor (RUNX) family comprises three members, RUNX1 (also designated AML-1, PEBP2åB, CBFA2), RUNX2 (also designated AML-3, PEBP2åA, CBFA1, Osf2) and RUNX3 (also designated AML-2, PEBPåC, CBFA3). RUNX family members are DNA-binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX2 is essential for skeletal mineralization in that it stimulates osteoblast differentiation of mesenchymal stem cells, promotes chondrocyte hypertrophy and contributes to endothelial cell migration and vascular invasion of developing bones. Regulating RUNX2 expression may be a useful therapeutic tool for promoting bone formation. Mutations in the C-terminus of RUNX2 are associated with cleidocranial dysplasia syndrome, an autosomal-dominant skeletal dysplasia syndrome that is characterized by widely patent calvarial sutures, clavicular hypoplasia, supernumerary teeth, and short stature.
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