|Application ||WB, IHC|
|Calculated MW||61993 Da|
|Positive Control||Western blot: HL60 cell line lysate, IHC: human hepatocarcinoma tissue.|
|Application & Usage||WB: 1:1000, IHC: 1:10 – 1:50.|
|Other Names||GNL3; E2IG3; NS; Guanine nucleotide-binding protein-like 3; E2-induced gene 3 protein; Novel nucleolar protein 47; Nucleolar GTP-binding protein 3; Nucleostemin|
|Formulation||This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Nucleostemin/GLN3 Antibody (CT) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).|
|Cellular Location||Nucleus. Nucleus, nucleolus. Note=Shuttles between the nucleus and nucleolus|
|Tissue Location||Increased levels in lung tissue in cancer patients.|
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Provided below are standard protocols that you may find useful for product applications.
Nucleostemin, also designated Nucleolar GTP-binding protein 3, is a member of the MMR1/HSR1 GTP-binding protein family. It is expressed in the nucleoli of adult CNS stem cells, primitive bone marrow cells, and embryonic stem cells and in several cancer cell lines. Nucleostemin is often used as a stem cell marker. Overexpression or depletion of the protein can reduce cell proliferation in CNS stem cells. Nucleostemin shuttles between the nucleus and the nucleolus and may be important in maintaining the proliferative capacity of stem cells. Nucleostemin is important in the growth regulation of liver cancer, gastric cancer and several other cancer types. The gene encoding Nucleostemin is localized to chromosome 3p21.1.
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