|Application ||WB, IF, FC|
|Clone Names||Clone # 8G3G10|
|Calculated MW||45183 Da|
|Positive Control||Western blot: mouse heart tissue lysate, IF: SY5Y cells, FACS: human brain tissue.|
|Application & Usage||WB: 1:500 – 1:2000, IHC: 1:50 – 1:100, IF: 1:100.|
|Other Names||CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR; NGFR|
|Formulation||This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||NGFR Antibody (Clone # 8G3G10) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
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NGFR, a 75 kd glycoprotein (also known as P-75NTR), is the first of neurotrophin receptors to be isolated and is a member of the tumor necrosis factor (TNF) receptor family. It is expressed not only in sympathetic and sensory neurons, but also in various neural crest cell or tumor derivatives such as melanocytes, melanomas, neuroblastomas, pheochromocytomas, neurofibromas, and neurotized nevi (type C melanocytes). NGFR has been shown to be a reliable marker for desmoplastic and neurotropic melanoma by several groups. It is now apparent that expression of NGFR is ubiquitous and not limited to the nervous system, being expressed in mature nonneural cells such as perivascular cells, follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells and myoepithelial cells. Studies in prostate and urothelial cancer suggest that NGFR may act as a tumour suppressor, negatively regulating cell growth and proliferation. Anti-NGFR labels the myoepithelial cells of breast ducts and intralobular fibroblasts of breast ducts and thus aides in the diagnosis of malignancy in the breast.
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