|Isotype||Mouse IgG1, 2bk|
|Clone Names||Clone # 7G7F9|
|Positive Control||Western blot: Ramos cell lysate.|
|Application & Usage||WB: 1:500 – 1:1000.|
|Other Names||HLA-A; HLAA; HLA class I histocompatibility antigen, A-3 alpha chain; MHC class I antigen A*3|
|Formulation||Crude ascites with 0.09% (W/V) sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||HLA-A Antibody (Clone # 7G7F9) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Major histocompatibility complex (MHC) molecules form an integral part of the immune response system. They are cell-surface receptors that bind peptides and present them to T lymphocytes. Human leukocyte antigens (HLAs) are polymorphic members of the MHC family that are specifically involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens: class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). Class I molecules are expressed in nearly all cells and play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. HLA-A encodes a membrane anchored heavy chain which hetero-dimerizes with a light chain (b-2-Microglobulin) to form MHC-I. Polymorphisms yield hundreds of HLA-A alleles.
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