Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   H3K9me3S10p Antibody   

H3K9me3S10p Antibody

Rabbit Polyclonal Antibody

  • CHIP - H3K9me3S10p Antibody ABV11343
    ChIP assays were performed using human HeLa cells treated with colcemid and the antibody and optimized PCR primer sets for qPCR. A titration of the antibody consisting of 2, 5, 10 and 15 μl per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative control. The Fig shows the recovery, expressed as a % of input (the relative amount of IP DNA compared to input DNA after qPCR analysis).
  • DB - H3K9me3S10p Antibody ABV11343
    A Dot Blot analysis was performed to test the cross reactivity of the antibody with peptides containing other modifications of histone H3 100 to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The Fig shows a high specificity of the antibody for the modification of interest.
  • E - H3K9me3S10p Antibody ABV11343
    To determine the titer, an ELISA was performed using a serial dilution of the antibody. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution the titer of the antibody was estimated to be 1:87,000.
  • WB - H3K9me3S10p Antibody ABV11343
    HeLa cells were treated with colcemid to block the cell cycle in metaphase and 15 μg were analysed by WB blot using the antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P68431
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 15404 Da
Additional Information
Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968
Positive Control IF: HeLa cells, WB: HeLa cells, ELISA: Antigen, ChIP: HeLa cells, Dot blot: Histone peptides
Application & Usage IF: 1:500, WB: 1:500, ELISA: 1:1000 – 1:10000, Dot Blot: 1:1000, ChIP: 5 µl/ChIP.
Other Names Histone H3
Target/Specificity H3K9me3S10p
Antibody Form Liquid
Appearance Colorless liquid
Formulation In PBS with 0.05% (W/V) sodium azide.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsH3K9me3S10p Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms H3FA
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

$ 245.00
Cat# ABV11343
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Santa Cruz Alternative
Terms & Conditions