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RARA polyclonal antibody

Rabbit Polyclonal Antibody

  • CHIP - RARA polyclonal antibody ABV11361
    ChIP assays were performed using NB4 cells and the antibody and optimized PCR primer sets for qPCR. A titration of the antibody consisting of 2, 5, 10, and 15 μl per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative control. The Fig shows the recovery, expressed as a % of input (the relative amount of IP DNA compared to input DNA after qPCR analysis).
  • E - RARA polyclonal antibody ABV11361
    To determine the titer, an ELISA was performed using a serial dilution of the antibody. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution the titer of the antibody was estimated to be 1:2400.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10276
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 50771 Da
Additional Information
Gene ID 5914
Positive Control Western blot: Human embryonic kidney cells, ELISA: peptides, ChIP: NB4 cells.
Application & Usage ChIP: 4 µg/ChIP, WB: 1:750, ELISA: 1:50.
Other Names Retinoic acid receptor alpha, Nuclear receptor subfamily 1 group B member 1, NR1B1
Target/Specificity RARA
Antibody Form Liquid
Appearance Colorless liquid
Formulation In PBS with 0.05% (W/V) sodium azide.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsRARA polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms NR1B1
Function Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand- dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.
Cellular Location Nucleus. Cytoplasm. Note=Nuclear localization depends on ligand binding, phosphorylation and sumoylation. Transloaction to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation
Research Areas
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RARA is a receptor for retinoic acid, a vitamin A metabolite, which directly regulates gene expression in target cells by binding to specific DNA response elements. In the absence of its ligand, this receptor represses transcription through the recruitment of specific corepressors and of HDAC’s, whereas binding of retinoic acid causes the recruitment of coactivators and HAT’s. Translocations involving the RARA gene, often leading to a RARA/PML fusion protein, are a major cause of acute promyelocytic leukemia.

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$ 245.00
Cat# ABV11361
(40 western blots)
Availability: 5-7days
Bulk Size
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