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MBD1 polyclonal antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9UIS9 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 66607 Da |
| Gene ID | 4152 |
|---|---|
| Positive Control | Western blot: Hela cells, ELISA: peptides, ChIP: U2OS cells. |
| Application & Usage | ChIP: 1.5 µg/ChIP, WB: 1:500, ELISA: 1:1000. |
| Other Names | CXXC3, PCM1, RFT, Methyl-CpG-Binding Domain 1 |
| Target/Specificity | MBD1 |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | In PBS with 0.05% sodium azide and 0.05% ProClin 300. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | MBD1 polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MBD1 (HGNC:6916) |
|---|---|
| Synonyms | CXXC3, PCM1 |
| Function | Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. |
| Cellular Location | Nucleus. Nucleus matrix. Nucleus speckle Chromosome Note=Nuclear, in a punctate pattern (PubMed:12711603). Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes (PubMed:10648624, PubMed:10454587). |
| Tissue Location | Widely expressed.. |
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Provided below are standard protocols that you may find useful for product applications.
Background
MBD1 is a transcriptional repressor that specifically binds to methylated CpG dinucleotides in promoter sequences. MBD1 acts by recruiting a variety of histone deacetylases (HDAC’s) and chromatin remodelling factors. MBD1-dependent transcriptional repression is mediated by ATF7IP through the recruitment of factors such as the histone methyltransferase SETDB1. MBD1 probably forms a complex with SETDB1 and ATF7IP which couples DNA methylation to H3K9 trimethylation and represses transcription.
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