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Runx1/AML1-ETO polyclonal antibody

Rabbit Polyclonal Antibody

  • CHIP - Runx1/AML1-ETO polyclonal antibody ABV11364
    ChIP assays were performed using Kasumi cells and the antibody and optimized PCR primer sets for qPCR. The Fig shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.
  • E - Runx1/AML1-ETO polyclonal antibody ABV11364
    To determine the titer, an ELISA was performed using a serial dilution of the antibody. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution the titer of the antibody was estimated to be 1:32,750.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q01196
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 48737 Da
Additional Information
Gene ID 861
Positive Control Western blot: SKNO-1 cells, ELISA: Peptides, ChIP: Kasumi cells.
Application & Usage ChIP: 4 µg/ChIP, WB: 1:1000, ELISA: 1:500
Other Names AML1-ETO
Target/Specificity Runx1/AML1-ETO
Antibody Form Liquid
Appearance Colorless liquid
Formulation In PBS with 0.05% (W/V) sodium azide.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsRunx1/AML1-ETO polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RUNX1
Synonyms AML1, CBFA2
Function CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B- dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down- regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532).
Cellular Location Nucleus.
Tissue Location Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood
Research Areas
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This antibody specifically recognizes the AML1 (RUNX1) - ETO (RUNX1T1) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)). This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.

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$ 245.00
Cat# ABV11364
(40 western blots)
Availability: 5-7days
Bulk Size
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