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PPARG polyclonal antibody

Rabbit Polyclonal Antibody

     
  • CHIP - PPARG polyclonal antibody ABV11368-25
    ChIP was performed on macrophages derived from mouse bone marrow using the antibody and optimized PCR primer sets for qPCR. Sheared chromatin from 1 million cells and 1 μg of PPARg antibody were used per ChIP experiment. IgG was used as a negative IP control. Figure 1: recovery, expressed as the % of input, of the PDK4 PPAR response element (RE).
  • CHIP - PPARG polyclonal antibody ABV11368-25
    ChIP was performed on macrophages derived from mouse bone marrow using the antibody and optimized PCR primer sets for qPCR. Sheared chromatin from 1 million cells and 1 μg of PPARg antibody were used per ChIP experiment. IgG was used as a negative IP control. Figure 2: recovery of the FABP4 Adipo PPAR RE in cells treated with RSG, a very strong activating ligand of PPARG, and in untreated cells.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P37231
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 57620 Da
Additional Information
Gene ID 5468
Positive Control Western blot: 293T cells, ELISA: Peptides, ChIP: mouse BM macrophages.
Application & Usage ChIP: 1 µg/ChIP, Western Blot: 1:2000, ELISA: 1:1000.
Other Names PPARG, NR1C3
Target/Specificity PPARG
Antibody Form Liquid
Appearance Colorless liquid
Formulation In PBS with 0.05% sodium azide and 0.05% ProClin 300.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsPPARG polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPARG
Synonyms NR1C3
Function Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity).
Cellular Location Nucleus. Cytoplasm. Note=Redistributed from the nucleus to the cytosol through a MAP2K1/MEK1-dependent manner NOCT enhances its nuclear translocation
Tissue Location Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
Research Areas
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Background

PPARG is a nuclear hormone receptor which binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Like many other nuclear hormone receptors, PPARG forms a heterodimer with the retinoid X receptor (RXR) leading to transcriptional regulation of various genes including acyl-CoA oxidase and cytochrome P450 A6. PPARG has been implicated in adipocyte differentiation and glucose homeostasis and in various diseases such as obesity, diabetes, atherosclerosis and cancer.

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$ 245.00
Cat# ABV11368-25
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