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ETO polyclonal antibody

Rabbit Polyclonal Antibody

     
  • CHIP - ETO polyclonal antibody ABV11369-50
    ChIP assays were performed using SKNO-1 cells, the antibody and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used.
  • E - ETO polyclonal antibody ABV11369-50
    An ELISA was performed using a serial dilution of the antibody. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1300.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
E
Primary Accession Q06455
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 67566 Da
Additional Information
Gene ID 862
Positive Control ELISA: Peptides, ChIP: SKNO-1 cells.
Application & Usage ChIP: 4 µl/ChIP, ELISA: 1:100.
Other Names RUNX1T1, AML1T1, CBFA2T1, CDR, MTG8, ZMYND2
Target/Specificity ETO
Antibody Form Liquid
Appearance Colorless liquid
Formulation In PBS with 0.05% (W/V) sodium azide.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsETO polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RUNX1T1
Synonyms AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2
Function Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:12559562, PubMed:15203199). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed:23251453). Can repress transactivation mediated by TCF12 (PubMed:16803958). Acts as a negative regulator of adipogenesis (By similarity). The AML1-MTG8/ETO fusion protein frequently found in leukemic cells is involved in leukemogenesis and contributes to hematopoietic stem/progenitor cell self-renewal (PubMed:23812588).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00440, ECO:0000269|PubMed:10973986}. Note=Colocalizes with ATN1 in discrete nuclear dots
Tissue Location Most abundantly expressed in brain. Lower levels in lung, heart, testis and ovary
Research Areas
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Background

ETO is a transcriptional regulator which belongs to the myeloid translocation gene family. ETO exerts its function by interaction with transcription factors bound to promoters and binding to histone deacetylases. It recruits a range of corepressors to facilitate transcriptional repression. The t (8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. This translocation produces a chimeric gene made up of the 5’-region of AML1 and the 3’-region of the ETO gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.

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$ 245.00
Cat# ABV11369-50
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