|Application ||WB, E|
|Calculated MW||68723 Da|
|Positive Control||Western blot: mouse fibroblasts and neural stem cells, ELISA: Peptides, IF: NIH3T3 cells.|
|Application & Usage||ELISA: 1:100 – 1:500, Western Blotting: 1:500 – 1:1000, IF: 1:200.|
|Other Names||ASH2L, ASH2L1, ASH2L2, Bre2|
|Formulation||In PBS with 0.05% (W/V) sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||ASH2 polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.|
|Tissue Location||Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes|
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Provided below are standard protocols that you may find useful for product applications.
Ash2 is a component of the Set1/Ash2 histone methyltransferase (HMT) complex. This complex specifically methylates K4 of histone H3, thereby activating transcription. Methylation of K4 is blocked by premethylation of the neighboring K9, a repressor of transcription. This indicates that the Set1/Ash2 HMT complex mediates the crosstalk between K9 methylation and K4 methylation. Ash2 plays a role in hematopoiesis and may be associated with some kinds of leukemia.
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