|Application ||WB, E|
|Calculated MW||141466 Da|
|Positive Control||Western blot: HeLa cells, ELISA: Peptides.|
|Application & Usage||ELISA: 1:100 – 1:200, Western Blotting: 1:500.|
|Other Names||GLP1, KMT1D|
|Formulation||In PBS with 0.05% (W/V) sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||EHMT1 polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||EUHMTASE1, GLP, KIAA1876, KMT1D|
|Function||Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non- histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.|
|Cellular Location||Nucleus. Chromosome. Note=Associates with euchromatic regions|
|Tissue Location||Widely expressed.|
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EHMT1 is a histone methyltransferase which specifically methylates histone H3 on lysine 9. H3K9 methylation represents a marker for epigenetic transcriptional repression. This repression is established by recruiting HP1 proteins to the methylated histone. H3K9 methylation may contribute to silencing of MYC and E2F responsive genes and plays a role in murine embryogenesis.
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