|Calculated MW||40721 Da|
|Positive Control||Western blot: HeLa cells|
|Application & Usage||Western Blot: 1:1000|
|Other Names||SET7, SET7/9, KMT7, H3-K4-HMTase|
|Formulation||In PBS with 0.05% sodium azide and 0.05% ProClin 300.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Set9 polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||KIAA1717, KMT7, SET7, SET9|
|Function||Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.|
|Cellular Location||Nucleus. Chromosome.|
|Tissue Location||Widely expressed. Expressed in pancreatic islets|
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Provided below are standard protocols that you may find useful for product applications.
Set9 is a histone methyltransferase which specifically methylates lysine 4 of histone H3 being a specific tag for epigenetic transcriptional activation. Further, methylation of histone H3K4 by Set9 precludes deacetylation of H3 by the NuRD complex and impairs Suv39h1-mediated methylation of H3K9. Set9 also methylates the tumor suppressor p53 at lysine 372, thereby enhancing its stability. It contains a SET domain but lacks the pre- and post-SET domains).
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