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SETD8 polyclonal antibody

Rabbit Polyclonal Antibody

     
  • WB - SETD8 polyclonal antibody ABV11379-25
    Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 20 μg) using the antibody diluted 1:1000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the right; the location of the protein of interest is indicated by an arrow.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9NQR1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 42890 Da
Additional Information
Gene ID 387893
Positive Control Western blot: HeLa cells
Application & Usage Western Blot: 1:1000.
Other Names SET07, SET8, PR/SET07, KMT5A, PR-Set7
Target/Specificity SETD8
Antibody Form Liquid
Appearance Colorless liquid
Formulation In PBS with 0.05% sodium azide and 0.05% ProClin 300.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsSETD8 polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KMT5A (HGNC:29489)
Function Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins (PubMed:12086618, PubMed:12121615, PubMed:15964846, PubMed:17707234, PubMed:27338793). Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1) (PubMed:12086618, PubMed:12121615, PubMed:15964846, PubMed:27338793, PubMed:15200950, PubMed:15933069, PubMed:15933070, PubMed:16517599). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression (PubMed:12086618, PubMed:12121615, PubMed:15964846, PubMed:15200950, PubMed:15933069, PubMed:15933070, PubMed:16517599). Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes (PubMed:12086618, PubMed:12121615, PubMed:15964846, PubMed:15200950, PubMed:15933069, PubMed:15933070, PubMed:16517599). Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis (PubMed:12086618, PubMed:12121615, PubMed:15964846, PubMed:15200950, PubMed:15933069, PubMed:15933070, PubMed:16517599). Involved in chromosome condensation and proper cytokinesis (PubMed:12086618, PubMed:12121615, PubMed:15964846, PubMed:15200950, PubMed:15933069, PubMed:15933070, PubMed:16517599). Nucleosomes are preferred as substrate compared to free histones (PubMed:12086618, PubMed:12121615, PubMed:15964846, PubMed:15200950, PubMed:15933069, PubMed:15933070, PubMed:16517599). Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes (PubMed:17707234). Plays a negative role in TGF- beta response regulation and a positive role in cell migration (PubMed:23478445).
Cellular Location Nucleus. Chromosome. Note=Specifically localizes to mitotic chromosomes (PubMed:12208845). Colocalized with SIRT2 at mitotic foci (PubMed:23468428). Associates with chromosomes during mitosis; association is increased in a H(2)O(2)-induced oxidative stress- dependent manner (PubMed:23468428). Associates with silent chromatin on euchromatic arms (PubMed:12086618). Not associated with constitutive heterochromatin (PubMed:12086618).
Citations (0)
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Background

SETD8 is a histone methyl transferase that specifically monomethylates ‘Lys-20’ of histone H4. This histone modification represents a specific tag for epigenetic transcriptional repression is enriched during mitosis. SETD8 may also play a role in maintaining silent chromatin by preventing the acetylation of the H4 tail.

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Discontinued
Cat# ABV11379-25
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