KDM1B polyclonal antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| E |
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Primary Accession | Q8NB78 |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 92098 Da |
Gene ID | 221656 |
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Positive Control | ELISA: Peptides. |
Application & Usage | ELISA: 1:500. |
Other Names | AOF1, LSD2, C6orf193 |
Target/Specificity | KDM1B |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | In PBS with 0.05% (W/V) sodium azide. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | KDM1B polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | KDM1B (HGNC:21577) |
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Function | Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of GLYR1 to achieve such activity, they form a multifunctional enzyme complex that modifies transcribed chromatin and facilitates Pol II transcription through nucleosomes (PubMed:30970244). |
Cellular Location | Nucleus. Chromosome. Note=Found in actively RNAPolII- transcribed gene bodies. |
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Provided below are standard protocols that you may find useful for product applications.
Background
KDM1B is a histone demethylase that demethylates lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. KDM1B is able to demethylate both mono- and di-methylated lysine 4 of histone H3, but has no effect on tri-methylated lysine 4, mono-, di- or tri-methylated lysine 9, mono-, di- or tri-methylated lysine 27, mono-, di- or tri-methylated lysine 36 of histone H3, or on mono-, di- or tri-methylated lysine 20 of histone H4. KDM1B is also required for de novo DNA methylation of a subset of imprinted genes during oogenesis. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.
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