|Application ||WB, E|
|Calculated MW||44597 Da|
|Positive Control||Western blot: NIH3T3 and embryonic stem cells, ELISA: Peptides.|
|Application & Usage||Western Blot: 1:1000, ELISA: 1:100 – 1:1000|
|Other Names||TRDMT1, M.HsaIIP, PuMet, RNMT1|
|Formulation||In PBS with 0.05% (W/V) sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||DNMT2 polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).|
|Tissue Location||Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes|
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Provided below are standard protocols that you may find useful for product applications.
Dnmt2 belongs to the family of DNA cytosine methyltransferases. Dnmt2, however, lacks the large N-terminal regulatory domain common to other eukaryotic methyltransferases and does not methylate CpG in genomic DNA. Instead, it specifically methylates cytosine 38 in the anticodon loop of Asp transfer RNA.
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