|Application ||WB, IP|
|Calculated MW||83884 Da|
|Positive Control||Western blot: HeLa cells, IP: HeLa cells|
|Application & Usage||Western Blot: 1:1000, IP: 1 - 5 µg/IP|
|Other Names||L3MBTL, L3MBT|
|Formulation||In PBS with 0.05% sodium azide and 0.05% ProClin 300.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||L3MBTL1 polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||KIAA0681, L3MBT, L3MBTL|
|Function||Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post- translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.|
|Cellular Location||Nucleus. Note=Excluded from the nucleolus. Does not colocalizes with the PcG protein BMI1, suggesting that these two proteins do not belong to the same complex|
|Tissue Location||Widely expressed. Expression is reduced in colorectal cancer cell line SW480 and promyelocytic leukemia cell line HL-60.|
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L3MBTL1 is a member of the polycomb group (PcG) proteins, which function as transcriptional regulators in large protein complexes. In Drosophila, homozygous mutations in the L3MBTL1 gene have been demonstrated to cause brain tumors, identifying L3MBTL1 as a tumor suppressor gene. L3MBTL1 forms a homodimer and interacts with TEL (ETV6). L3MBTL1 probably plays a role in cell proliferation. Overexpression of L3MBTL1 induces multinucleated cells, suggesting that it is required for normal mitosis.
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