|Application ||WB, IF|
|Calculated MW||22225 Da|
|Positive Control||Western blot: HeLa cells, IF: HeLa cells.|
|Application & Usage||Western Blot: 1:1000, IF: 1:500|
|Other Names||HP1α, HP1A|
|Formulation||In PBS with 0.05% sodium azide and 0.05% ProClin 300.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||HP1α polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.|
|Cellular Location||Nucleus. Chromosome. Chromosome, centromere Note=Component of centromeric and pericentromeric heterochromatin Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase|
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Provided below are standard protocols that you may find useful for product applications.
CBX5/HP1 alpha is a component of heterochromatin. It recognizes and binds to histone H3 tails methylated at ‘Lys-9’, leading to epigenetic repression of transcription. HP1 alpha may also interact with lamin B receptor (LBR), thereby contributing to the association of the heterochromatin with the inner nuclear membrane. Further, HP1 alpha is involved in the formation of a functional kinetochore through interaction with MIS12 complex proteins.
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