HP1α polyclonal antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF |
---|---|
Primary Accession | P45973 |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 22225 Da |
Gene ID | 23468 |
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Positive Control | Western blot: HeLa cells, IF: HeLa cells. |
Application & Usage | Western Blot: 1:1000, IF: 1:500 |
Other Names | HP1α, HP1A |
Target/Specificity | HP1α |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | In PBS with 0.05% sodium azide and 0.05% ProClin 300. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | HP1α polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CBX5 |
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Synonyms | HP1A |
Function | Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. |
Cellular Location | Nucleus. Chromosome. Chromosome, centromere Note=Colocalizes with HNRNPU in the nucleus (PubMed:19617346) Component of centromeric and pericentromeric heterochromatin Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase (PubMed:19617346) Localizes to sites of DNA damage (PubMed:28977666) |
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Provided below are standard protocols that you may find useful for product applications.
Background
CBX5/HP1 alpha is a component of heterochromatin. It recognizes and binds to histone H3 tails methylated at ‘Lys-9’, leading to epigenetic repression of transcription. HP1 alpha may also interact with lamin B receptor (LBR), thereby contributing to the association of the heterochromatin with the inner nuclear membrane. Further, HP1 alpha is involved in the formation of a functional kinetochore through interaction with MIS12 complex proteins.
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