|Application ||WB, IF|
|Other Accession||P83916, Q13185|
|Calculated MW||22225 Da|
|Positive Control||ChIP: NIH 3T3 cells, Western blot: HeLa cells, IF: HeLa cells.|
|Application & Usage||Western Blot: 1:1000, IF: 1:500, ChIP: 4 µg/ChIP|
|Other Names||CBX5, 1, 3|
|Target/Specificity||HP1α, ß and γ|
|Formulation||In PBS with 0.05% sodium azide and 0.05% ProClin 300.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||HP1α, ß and γ polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.|
|Cellular Location||Nucleus. Chromosome. Chromosome, centromere Note=Component of centromeric and pericentromeric heterochromatin Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase|
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Provided below are standard protocols that you may find useful for product applications.
HP1 alpha, beta and gamma are components of heterochromatin. They recognize and bind histone H3 tails methylated at ‘Lys-9’, leading to epigenetic repression of transcription. HP1 alpha, beta and gamma also interact with lamin B receptor (LBR), thereby contributing to the association of heterochromatin with the inner nuclear membrane.
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