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H3R17me2 polyclonal antibody

Rabbit Polyclonal Antibody

  • CHIP - H3R17me2 polyclonal antibody ABV11406
    ChIP assays were performed using 1 million U2OS cells and the antibody and optimized PCR primer sets for qPCR. A titration of the antibody consisting of 1, 2, 5, and 10 μl per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative control. The Fig shows the recovery, expressed as a % of input (the relative amount of IP DNA compared to input DNA after qPCR analysis).
  • DB - H3R17me2 polyclonal antibody ABV11406
    A Dot Blot analysis was performed to test the cross reactivity of the antibody with peptides containing other modifications of histone H3, H4 and an unmodified H3 sequence. 100 to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The Fig shows a high specificity of the antibody for the modification of interest.
  • E - H3R17me2 polyclonal antibody ABV11406
    To determine the titer, an ELISA was performed using a serial dilution of the antibody. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution the titer of the antibody was estimated to be 1:40,000.
  • WB - H3R17me2 polyclonal antibody ABV11406
    HeLa cells histone extracts (15 μg) were analysed by WB blot using the antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P68431
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 15404 Da
Additional Information
Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968
Positive Control Western blot: HeLa cells, ELISA: Antigen, ChIP: U2OS cells, Dot blot: Histone Peptides.
Application & Usage WB: 1:250, ELISA: 1:1000 – 1:3000, Dot Blot: 1:20,000, ChIP: 10-15 µl/ChIP.
Other Names Histone 3
Target/Specificity H3R17me2
Antibody Form Liquid
Appearance Colorless liquid
Formulation In PBS with 0.05% (W/V) sodium azide.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsH3R17me2 polyclonal antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms H3FA
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Citations (0)

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Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.

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$ 245.00
Cat# ABV11406
(40 western blots)
Availability: 5-7days
Bulk Size
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