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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Cyclin E1 Antibody   

Cyclin E1 Antibody

Rabbit Polyclonal Antibody

  • IF - Cyclin E1 Antibody ABV11443
    Immunofluorescent analysis of Cyclin E1 staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
  • IHC - Cyclin E1 Antibody ABV11443
    Immunohistochemical analysis of Cyclin E1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • WB - Cyclin E1 Antibody ABV11443
    Western blot analysis of Cyclin E1 expression in HEK293T (A), mouse brain (B), PC12 (C), SW626 (D), HeLa (E), K562 (F) whole cell lysates.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P24864
Reactivity Human, Mouse, Rat, Pig, Bovine, Dog
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 47077 Da
Additional Information
Gene ID 898
Positive Control WB: HEK293T, mouse brain, PC12, SW626, HeLa, K562, whole cell lysates, IHC: human lung cancer, IC/IF: PC12 cells
Application & Usage WB: 1:500 - 1:1000, IHC: 1:100 – 1:200, IF/IC: 1:100 – 1:500
Other Names CCNE1, Cyclin Es, Cyclin Et, G1/S-specific cyclin-E1
Target/Specificity Cyclin E1
Antibody Form Liquid
Appearance Colorless liquid
Formulation 1 mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsCyclin E1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCNE1
Synonyms CCNE
Function Essential for the control of the cell cycle at the G1/S (start) transition.
Cellular Location Nucleus.
Tissue Location Highly expressed in testis and placenta. Low levels in bronchial epithelial cells
Research Areas
Citations (0)

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Cyclin E1 is a regulatory subunit of the CDK2 kinase. It is central for the regulation of the G1/S transition and its abundance is tightly regulated throughout the cell cycle via ubiquitination. Cyclin E has been the subject of intense study in relation to tumorigenesis and cancer management and prognosis. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

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$ 190.00
Cat# ABV11443
(40 western blots)
Availability: 5-7days
Bulk Size
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