|Application ||WB, IHC, IF, ICC|
|Reactivity||Human, Mouse, Rat, Pig, Bovine, Dog|
|Calculated MW||47077 Da|
|Positive Control||WB: HEK293T, mouse brain, PC12, SW626, HeLa, K562, whole cell lysates, IHC: human lung cancer, IC/IF: PC12 cells|
|Application & Usage||WB: 1:500 - 1:1000, IHC: 1:100 – 1:200, IF/IC: 1:100 – 1:500|
|Other Names||CCNE1, Cyclin Es, Cyclin Et, G1/S-specific cyclin-E1|
|Formulation||1 mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Cyclin E1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential for the control of the cell cycle at the G1/S (start) transition.|
|Tissue Location||Highly expressed in testis and placenta. Low levels in bronchial epithelial cells|
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Provided below are standard protocols that you may find useful for product applications.
Cyclin E1 is a regulatory subunit of the CDK2 kinase. It is central for the regulation of the G1/S transition and its abundance is tightly regulated throughout the cell cycle via ubiquitination. Cyclin E has been the subject of intense study in relation to tumorigenesis and cancer management and prognosis. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
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