|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||87640 Da|
|Positive Control||WB: HEK293T, mouse liver, rat liver, whole cell lysates, IHC: human breast cancer|
|Application & Usage||WB: 1:500 - 1:1000, IHC: 1:100 – 1:200.|
|Other Names||F-box only protein 1, CCNF, FBX1, FBXO1, G2/mitotic-specific cyclin-F|
|Formulation||1 mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Cyclin F Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication.|
|Cellular Location||Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Note=Localization in the centrosome is rare in S phase cells and increases in G2 cells, Localizes on both the mother and daughter centrioles. Localization to centrosomes is not dependent on CP110 Also localizes to the nucleus|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
Cyclin F is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication. It is ubiquitously expressed in human cells but fluctuates dramatically through the cell cycle, peaking in G2 like cyclin A and decreasing prior to decline of cyclin B. Cyclin F exhibits regulated subcellular localization, being localized in the nucleus in most cells, with a significant percentage of cells showing only perinuclear staining.
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