|Application ||WB, IF, ICC|
|Calculated MW||18930 Da|
|Positive Control||WB: CCRFCEM, Jurkat, HuT78 whole cell lysates, IF/IC: Jurkat cells|
|Application & Usage||WB: 1:500 - 1:1000, IF/IC: 1:100 – 1:500|
|Other Names||T3D; T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; CD3d|
|Formulation||1 mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||CD3d Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The CD3 complex mediates signal transduction.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
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T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma,CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine based activation motifs (ITAMs). Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK cell-positive (T-/B+/NK+SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.
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