|Reactivity||Human, Mouse, Rat, Monkey, Dog|
|Calculated MW||51111 Da|
|Positive Control||WB: SP20, PC12, H9C2 whole cell lysate|
|Application & Usage||WB: 1:500 - 1:1000|
|Other Names||T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4|
|Formulation||1 mg/ml in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||CD4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum|
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Provided below are standard protocols that you may find useful for product applications.
CD4 is a cell-surface glycoprotein found on the mature helper T cells and immature thymocytes, as well as on monocytes and macrophages. (Some cytotoxic T cells have CD4 protein as well.) Normally, about 65% of T cells in the blood are CD4+ (have CD4 protein protruding from their membrane). A mature T cell with have either CD4 or CD8, but not both. During one stage of development T cells develop CD4 and CD8 receptors, but they eventually are differentiated in the thymus and become more specialized. CD4 is also expressed on cortical cells, mature medullary thymocytes, microglial cells and dendritic cells.
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