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S- Adenosylhomocysteine Antibody (Clone # 301-10)

Mouse Monoclonal Antibody

  • E - S- Adenosylhomocysteine Antibody (Clone # 301-10) ABV11456-25
    Competitive ELISA: 0.5 μg/ml of SAH-BNSA coating standard was coated into 96 wells. Serial dilution of SAH standard, S-Adenosylmethionine (SAM), Adenosine, Homocysteine, L-cysteine, Glutathione, L-Cystathionine, and antibody were added. HRP conjugated Goat anti-Mouse IgG antibody was used to develop the color. The A is the OD450 value of the test well and the A0 is the OD450 of the well without competitive antigen.
  • IHC - S- Adenosylhomocysteine Antibody (Clone # 301-10) ABV11456-25
    Immunohistochemistry staining was performed using the antibody with benign kidney tissue adjacent to carcinoma. Brown areas indicated strong positive staining in cytoplasm (X400).
  • IHC - S- Adenosylhomocysteine Antibody (Clone # 301-10) ABV11456-25
    The immunohistochemical staining was performed for the same sample as in the above figure with kidney cancer tissue. Cytoplasm showed background staining (further dilution beyond 1:200 is required) with the antibody (X400).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity All Species
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG3
Clone Names 301-10
Additional Information
Positive Control IHC: kidney carcinoma tissue, FCM: HepG2 and L02 cell lines
Application & Usage cELISA: 1:2500, FCM: 1: 20/40, IHC: 1: 20/40.
Other Names S- Adenosylhomocysteine
Target/Specificity S Adenosylhomocysteine
Antibody Form Liquid
Appearance Colorless liquid
Formulation 20 mM PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide, 50% Glycerol and 10 mg/ml BSA
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsS- Adenosylhomocysteine Antibody (Clone # 301-10) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)

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S-Adenosyl-L-homocysteine (SAH) is an amino acid derivative and an intermediate, by-product, or modulator of several metabolic pathways, including the activated methyl cycle and cysteine biosynthesis. It is also a product of S-adenosyl-methionine (SAM)-dependent methylation of biological molecules, including DNA, RNA, and histones and other proteins. SAH is a risk factor for many diseases, including cancer and neurodegenerative diseases.

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$ 155.00
Cat# ABV11456-25
Availability: 5-7days
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