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LC3 (APG8A) (NT) Antibody

Rabbit Polyclonal Antibody

     
  • IF - LC3 (APG8A) (NT) Antibody ABV11459-100
    Fluorescent image of U251 cells stained with LC3 (APG8A) (N-term) antibody. U251 cells were treated with Chloroquine (50 μM, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with LC3 (APG8A) (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
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  • IHC - LC3 (APG8A) (NT) Antibody ABV11459-100
    Formalin-fixed and paraffin-embedded human brain tissue reacted with Autophagy LC3 Antibody (APG8a) (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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  • WB - LC3 (APG8A) (NT) Antibody ABV11459-100
    Western blot analysis of anti-LC3 (APG8a) Pab in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).
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  • WB - LC3 (APG8A) (NT) Antibody ABV11459-100
    APG8a (MAP1LC3A) Antibody (M1) western blot analysis in mouse brain tissue lysates (35ug/lane). This demonstrates the APG8a (MAP1LC3A) antibody detected the APG8a (MAP1LC3A) protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession Q9H492
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 14272 Da
Additional Information
Gene ID 84557
Positive Control WB: rat brain lysate, IHC: human brain tissue, IF: U251 cells
Application & Usage WB: 1:1000, IF: 1:200, IHC: 1:50-100.
Other Names MAP1LC3A; Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; Microtubule-associated protein 1 light chain 3 alpha
Target/Specificity LC3
Antibody Form Liquid
Appearance Colorless liquid
Formulation Supplied in PBS with 0.09% (W/V) sodium azide.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsLC3 (APG8A) (NT) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP1LC3A
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538, PubMed:31006537).
Cellular Location Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Endomembrane system; Lipid-anchor. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q91VR7}. Note=LC3-II binds to the autophagic membranes.
Tissue Location Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes
Research Areas
Citations (0)
citation

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Background

Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.

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Discontinued
Cat# ABV11459-100
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