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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   CD45RA FITC Monoclonal Antibody (Clone HI100)   

CD45RA FITC Monoclonal Antibody (Clone HI100)

Mouse Monoclonal Antibody

  • FC - CD45RA FITC Monoclonal Antibody (Clone HI100) ABV11477
    Human peripheral blood lymphocytes were stained with FITC HI100 with relevant isotype control in Red.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08575
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG2b, Kappa
Clone Names HI100
Calculated MW 147254 Da
Additional Information
Gene ID 5788
Positive Control FACS: Human peripheral blood lymphocytes
Application & Usage Flow (Cell Surface): 5 µl/1x10^6 cells, Volume per test: 5 µl (1 µg).
Other Names CD45RA
Target/Specificity CD45RA
Antibody Form Liquid
Appearance Colorless liquid
Formulation Phosphate-buffered aqueous solution pH 7.2, ≤0.09% Sodium azide, may contain carrier protein/stabilizer.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage 4°C
Background Descriptions
PrecautionsCD45RA FITC Monoclonal Antibody (Clone HI100) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms CD45
Function Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein Membrane raft. Note=Colocalized with DPP4 in membrane rafts
Research Areas
Citations (0)

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CD45, a transmembrane multifunctional glycoprotein, is a member of Type I receptor-linked PTPase family and is expressed as multiple isoforms due to alternative splicing. Expression of these isoforms is highly regulated and shift in this expression determines T-cell activation. CD45RB consists of exon B and is predominantly expressed in naïve T-cells secreting IL-2. Its expression is low in primed/memory T cells, cells that express Th2 cytokines such as IL-4 and IL-10 and population of T-cells with regulatory function. Immunotherapy with CD45RB antibody is being widely studied in transplantation and vaccination. CD45 antibodies are commonly used to identify tumors of lymphoid origin. The HI100 monoclonal antibody specifically reacts with human CD45RA, the 220 kDa isoform of the human leukocyte common antigen (LCA) found on 40-50% of the peripheral CD4+ T lymphocytes, half of the peripheral CD8+ T lymphocytes and some of the monocytes and B lymphocytes. The CD45RA antigen is expressed by naïve and activated T lymphocytes. The HI100 monoclonal antibody is used as a phenotypic marker to discriminate T lymphocytes subsets.

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$ 105.00
$ 245.00
Cat# ABV11477
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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