|Application ||WB, IF|
|Calculated MW||77244 Da|
|Other Names||BIRC5, IAP4 , API4, SVV , EPR-1|
|Format||100 µg (0.5 mg/ml) affinity purified rabbit anti-Survivin polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Precautions||Survivin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro- apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).|
|Cellular Location||Cell membrane. Cytoplasm. Nucleus. Cell projection, growth cone. Nucleus speckle Note=Colocalizes with TSHZ3 in axonal growth cone (By similarity) In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via its interaction with APP. Following exposure to DNA damaging agents, it is released from cell membrane and translocates to the nucleus Nuclear translocation is under the regulation of APP. Colocalizes with TSHZ3 in the nucleus. Colocalizes with NEK6 at the nuclear speckles. Phosphorylation at Ser-610 by SGK1 promotes its localization to the nucleus (By similarity).|
|Tissue Location||Highly expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Survivin is a newly described apoptosis inhibitor that is expressed in many human cancers, but undetectable in terminally differentiated adult tissues. It has been shown that recombinant expression of Survivin counteracts apoptosis of B lymphocyte precursors deprived of interleukin 3 (IL-3), s µggesting a potential role of Survivin in cancer therapy. Survivin is expressed in G2-M phase in a cell cycle-dependent manner and directly associated with mitotic spindle microtubules, s µggesting a role in both apoptosis regulation and cell cycle progression.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.