NIK Antibody
Purified Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q99558 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 104042 Da |
Gene ID | 9020 |
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Other Names | Mitogen-activated protein kinase kinase kinase 14, 2.7.11.25, NF-kappa-beta-inducing kinase, HsNIK, Serine/threonine-protein kinase NIK, MAP3K14, NIK |
Target/Specificity | NIK |
Formulation | 100 µg (0.2 mg/ml) affinity purified rabbit anti-NIK polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Background Descriptions | |
Precautions | NIK Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MAP3K14 (HGNC:6853) |
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Function | Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Phosphorylates CHUK/IKKA, thereby promoting proteolytic processing of NFKB2/P100, which leads to NF-kappa-B activation via the non-canonical pathway (PubMed:25406581, PubMed:29230214). Has an essential role in the non-canonical NF-kappa-B signaling that regulates genes encoding molecules involved in B-cell survival, lymphoid organogenesis, and immune response (PubMed:25406581). Could act in a receptor-selective manner. |
Cellular Location | Cytoplasm. |
Tissue Location | Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon |
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Provided below are standard protocols that you may find useful for product applications.
Background
NIK (NF-kB-inducing kinase) is a member of the MAP kinase kinase kinase family that binds TRAF2 and stimulates NF-kB activity. NIK was initially isolated from a human B cell cDNA library and contains 795 amino acids with an apparent molecular weight of slightly more than 97 kDa on SDS gel. NIK is a serine/threonine kinase and its kinase activity contributes to IkB phosphorylation. The carboxyl terminal segment of NIK binds TRAF2. A mutant NIK with intact carboxyl terminus but without the two lysine residues at its catalytic domain serves as a dominant-negative inhibitor for NF-kB activation. NIK also interacts with TRAF6 and mediates IL-1-induced NF-kB activation.
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