|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||58582 Da|
|Other Names||KAT5 Antibody: lysine acetyltransferase 5, K-acetyltransferase 5, lysine acetyltransferase 5, HIV-1 Tat interactive protein 60kDa, HTATIP, HTATIP1, TIP, cPLA2 interacting protein, cPLA2, ESA1, PLIP|
|Formulation||1 mg/ml in PBS containing 0.02% sodium azide|
|Handling||The antibody solution should be gently mixed before use.|
|Precautions||TIP60 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation. Relieves NR1D2-mediated inhibition of APOC3 expression by acetylating NR1D2. Promotes FOXP3 acetylation and positively regulates its transcriptional repressor activity (PubMed:17360565).|
|Cellular Location||Nucleus. Nucleus, nucleolus. Cytoplasm, perinuclear region. Note=Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region and UV irradiation induces translocation into punctuate subnuclear structures named nuclear bodies|
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TIP60 belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and non-histone proteins. TIP60 has been shown to play a role in DNA repair and apoptosis and is thought to play an important role in signal transduction and autophagy. In case of HIV-1 infection, interaction with the viral Tat protein leads to TIP60 polyubiquitination and targets it to degradation. Also, TIP60 contains a chromodomain. TIP60 is a catalytic subunit of the NuA4 histone acetyltransferase complex. The NuA4 HAT complex plays a role in transcriptional activation of select genes mainly by acetylation of nucleosomal histone H4 and H2A, which influence nucleosome-DNA interaction and promotes interaction of the modified histones with other proteins that could regulate transcription positively. This complex also involves in the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, DNA repair and apoptosis.
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