Phospho-Bcl-x (Thr47) antibody
Purified Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, IF |
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Primary Accession | Q07817 |
Other Accession | Q64373 (Mouse), P53563 (Rat) |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 26049 Da |
Gene ID | 598 |
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Other Names | BCL2L1; BCL2L; BCLX; Bcl-2-like protein 1; Bcl2-L-1; Apoptosis regulator Bcl-X |
Target/Specificity | Bcl-x (Thr47) |
Formulation | 100 ug (1mg/ml) of antibody in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
Handling | The antibody solution should be gently mixed before use |
Background Descriptions | |
Precautions | Phospho-Bcl-x (Thr47) antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | BCL2L1 |
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Synonyms | BCL2L, BCLX |
Function | Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage- dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(S) promotes apoptosis. |
Cellular Location | [Isoform Bcl-X(L)]: Mitochondrion inner membrane. Mitochondrion outer membrane Mitochondrion matrix. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side. Note=After neuronal stimulation, translocates from cytosol to synaptic vesicle and mitochondrion membrane in a calmodulin-dependent manner (By similarity). Localizes to the centrosome when phosphorylated at Ser-49 |
Tissue Location | Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain |
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Provided below are standard protocols that you may find useful for product applications.
Background
Bcl-x is a dominant regulator of programmed cell death in mammalian cells. Members of the wider Bcl-2 family, which also includes Bcl-x, Bcl-w and Mcl-1, are described by their similarity to Bcl-2 protein. Bcl-x an antiapoptotic member of the Bcl-2 family is a dominant regulator of programmed cell death in mammalian cells. Bcl-x is located at the outer mitochondrial membrane and regulates outer mitochondrial membrane channel (VDAC) opening. The long form (Bcl-x (L)) displays cell death repressor activity, but the short isoform (Bcl-x(S)) and the b-isoform (Bcl-xb) promote cell death.
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