Anti-human CD9 antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, FC, E |
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Primary Accession | P21926 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Isotype | Mouse IgG1 |
Calculated MW | 25416 Da |
Gene ID | 928 |
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Other Names | CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, MRP-1, Tetraspanin-29, Tspan-29, p24, CD9, CD9, MIC3, TSPAN29 |
Target/Specificity | CD9 (unconjugated) |
Formulation | 1 mg/ml in phosphate buffered saline (PBS) with sodium azide (15 mM), Approx. pH: 7.4. |
Handling | The antibody solution should be gently mixed before use |
Background Descriptions | |
Precautions | Anti-human CD9 antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CD9 {ECO:0000303|PubMed:1840589, ECO:0000312|HGNC:HGNC:1709} |
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Function | Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:8478605, PubMed:14575715, PubMed:18541721). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480). Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed:18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed:8478605, PubMed:7511626). |
Cellular Location | Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Secreted, extracellular exosome {ECO:0000250|UniProtKB:P40240}. Note=Present at the cell surface of oocytes. Accumulates in the adhesion area between the sperm and egg following interaction between IZUMO1 and its receptor IZUMO1R/JUNO {ECO:0000250|UniProtKB:P40240} |
Tissue Location | Detected in platelets (at protein level) (PubMed:19640571). Expressed by a variety of hematopoietic and epithelial cells (PubMed:19640571). |
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Background
Anti-CD9 recognizes a human 24-kiloDalton (kDa) single-chain cell-surface glycoprotein (p24) belonging to the tetraspanin family which transverse the membrane four times. The CD9 antigen has a very broad tissue distribution. It is present on basophils, eosinophils, monocytes, pre-B cells, B cells, and various leukemic cell lines (erythroid, myeloid, some T-lymphoid, pre–B-lymphoid). It is also found on follicular center cells, sinus histocytes, macrophages, Kupffer cells, osteoclasts, hepatocytes, bile duct endothelium, renal glomeruli, proximal and distal tubuli, epithelia (intercellular spinous spaces) of skin and mucosa, fibroblasts, connective tissue, endothelium, smooth muscle, cardiac muscle, synovial lining cells, brain white matter, peripheral nerves and human keratin. In addition to being a co-stimulatory molecule, CD9 antigen activates platelets and induces mitogenesis and kinase activity and is involved in cell migration, cell adhesion, and integrin signaling.
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