|Application ||WB, IHC-P, IHC-F, FC, E, IP|
|Calculated MW||25637 Da|
|Other Names||CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63, CD63, MLA1, TSPAN30|
|Formulation||1 mg/ml in phosphate buffered saline (PBS) with sodium azide (15 mM), Approx. pH: 7.4.|
|Handling||The antibody solution should be gently mixed before use|
|Precautions||Anti-human CD63 antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.|
|Cellular Location||Cell membrane; Multi- pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi- pass membrane protein. Endosome, multivesicular body. Melanosome. Secreted, exosome. Cell surface. Note=Also found in Weibel-Palade bodies of endothelial cells (PubMed:10793155). Located in platelet dense granules (PubMed:7682577). Detected in a subset of pre- melanosomes. Detected on intralumenal vesicles (ILVs) within multivesicular bodies (PubMed:21962903)|
|Tissue Location||Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages|
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Provided below are standard protocols that you may find useful for product applications.
Anti-CD63 recognizes an extracellular fragment of CD63, a 56-kilodalton (kDa), type III lysosomal glycoprotein, belonging to the tetraspanin family. CD63 is expressed by granulocytes, platelets, T-cells, monocytes/macrophages, endothelial cells. CD63 protein is also expressed in exosome membrane. Cell sur-face exposition of CD63 is usually activation dependent. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell co-stimulator molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
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