JMJD2A Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | O75164 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 120662 Da |
Gene ID | 9682 |
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Positive Control | Western blot: DLD1 cell lysate |
Application & Usage | Western blot: 1:200 dilution. Not recommended for FACS and IHC based on probable cross reactivity with other JMJD containing proteins. |
Alias Symbol | JMJD2A |
Other Names | Jumonji Domain Containing 2A; KDM4A; Lysine-Specific Demethylase 4A |
Appearance | Colorless liquid |
Formulation | 100 µl of antibody in TBS, pH 7.4, containing 50% glycerol, 0.5 mg/ml BSA, and 0.02% sodium azide. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | JMJD2A Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | KDM4A |
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Synonyms | JHDM3A, JMJD2, JMJD2A, KIAA0677 |
Function | Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code (PubMed:26741168). Does not demethylate histone H3 'Lys- 4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. |
Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00537, ECO:0000269|PubMed:15927959, ECO:0000269|PubMed:16024779} |
Tissue Location | Ubiquitous.. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Jumonji Domain containing 2A (JMJD2A) is a lysine specific demethylase with emerging roles in histone modification or epigenetic remodeling. This JMJD2A polyclonal antibody was raised against an N-terminal recombinant fragment of JMJD2A. This fragment includes amino acids 1-350 including the JMJN and JMJC domains but not the two LAP/PHD zinc finger or Tudor domains of the 1,064 amino acid protein. JMJD2A is detected at 135 kDa by western blotting using a DLD1 cell lysate as a positive control. Also, the expression of JMJD2A varies by tissue, and northern blotting suggests skeletal muscle as a negative control and lung as a positive control tissue.
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