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Flag-Tag Monoclonal Antibody
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IP |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG |
| Application & Usage | Western blot: 1-5 µg/ml; Immunoprecipitation: 5 µg antibody for 0.5 mg total cell lysates. |
|---|---|
| Alias Symbol | DYKDDDDK |
| Other Names | FLAG, DYKDDDDK |
| Appearance | Colorless liquid |
| Formulation | 100 ug (1mg/ml) of antibody in 0.01M Tris-HCl, pH 8.0, 0.15M NaCl, and 0.02% sodium azide. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | Flag-Tag Monoclonal Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
DYKDDDDK-Tag (Flag-Tag) antibody detects over-expressed proteins containing the D epitope tag fused to either the amino- or carboxy-terminus of targeted proteins. Epitope tags are usually for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. The DYKDDDK epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian systems.
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