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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Flag-Tag Monoclonal Antibody   

Flag-Tag Monoclonal Antibody

Mouse Monoclonal Antibody

     
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  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IP
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG
Additional Information
Application & Usage Western blot: 1-5 µg/ml; Immunoprecipitation: 5 µg antibody for 0.5 mg total cell lysates.
Alias Symbol DYKDDDDK
Other Names FLAG, DYKDDDDK
Appearance Colorless liquid
Formulation 100 ug (1mg/ml) of antibody in 0.01M Tris-HCl, pH 8.0, 0.15M NaCl, and 0.02% sodium azide.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsFlag-Tag Monoclonal Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)

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Background

DYKDDDDK-Tag (Flag-Tag) antibody detects over-expressed proteins containing the D epitope tag fused to either the amino- or carboxy-terminus of targeted proteins. Epitope tags are usually for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. The DYKDDDK epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian systems.

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$ 295.00
Cat# ABV11740-100
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