Prothrombin Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
---|---|
Primary Accession |  , P00734 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Positive Control | WB: h Prothrombin, h serum |
---|---|
Application & Usage | WB: 1-4 µg |
Alias Symbol | F2 |
Other Names | Coagulation factor II |
Appearance | Colorless liquid |
Formulation | In PBS pH 7.2, 0.01 % BSA, 0.03 % ProClin® and 50 % glycerol |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Prothrombin Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Prothrombin is a vitamin K-dependent plasma protein which is synthesized in the liver. Prior to secretion into plasma, prothrombin undergoes post-translational modification by a vitamin K-dependent carboxylase which converts ten specific glutamic acid residues to γ-carboxyglutamic acid (gla). Conversion to thrombin is a key step in the blood coagulation pathway and catalyzes the coagulation of fibrinogen. Clinically, cases of selective deficiency are rare, although, in cases of liver cirrhosis, prothrombin is decreased. During activation, prothrombin is cleaved at Arg271-Thr272 and at Arg320-Ser321 to a "pro" fragment (fragment 1.2) and thrombin, the latter of which is composed of two chains covalently linked by a disulfide bond. There is an additional thrombin feed-back cleavage at Arg284-Thr285 resulting in an additional 13 amino acids being removed from the mature thrombin “A” chain.
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