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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Glycine Oxidase Antibody   

Glycine Oxidase Antibody

Rabbit Polyclonal Antibody

     
  • WB - Glycine Oxidase Antibody ABV11810-100
    Western blot analysis of Glycine Oxidase: Lane1: r Glycine Oxidase, 2ng; Lane2: r Glycine Oxidase, 10ng; Lane3: r Glycine Oxidase, 50ng;
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O31616
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 40937 Da
Additional Information
Gene ID 939377
Positive Control WB: r Glycine Oxidase
Application & Usage WB: 1-4 µg
Alias Symbol thiO
Other Names Glycine oxidase, glycine oxygen oxidoreductase (deaminating), GO
Appearance Colorless liquid
Formulation In PBS pH 7.2, 0.01 % BSA, 0.03 % ProClin® and 50 % glycerol
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsGlycine Oxidase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name thiO {ECO:0000303|PubMed:12627963}
Function Catalyzes the FAD-dependent oxidative deamination of various amines and D-amino acids to yield the corresponding alpha- keto acids, ammonia/amine, and hydrogen peroxide. Oxidizes sarcosine (N-methylglycine), N-ethylglycine and glycine (PubMed:9827558, PubMed:11744710, PubMed:19864430). Can also oxidize the herbicide glyphosate (N-phosphonomethylglycine) (PubMed:19864430). Displays lower activities on D-alanine, D- valine, D-proline and D-methionine (PubMed:9827558, PubMed:11744710). Does not act on L-amino acids and other D-amino acids (PubMed:9827558). Is essential for thiamine biosynthesis since the oxidation of glycine catalyzed by ThiO generates the glycine imine intermediate (dehydroglycine) required for the biosynthesis of the thiazole ring of thiamine pyrophosphate (PubMed:12627963).
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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Background

Glycine oxidase (GO) from Bacillus subtilis (EC 1.4.3.19) is a homotetrameric flavin-dependent oxidoreductase. Each GO monomer is non-covalently bound to flavin adenine dinucleotide. GO catalyzes oxidative deamination of various primary and secondary amines (e.g. glycine, sarcosine, N-ethylglycine) and some D-amino acids (e.g. D -alanine, D -proline, D -valine) to the corresponding α-keto acids and hydrogen peroxide. Primarily, glycine oxidase catalyzes the oxidation of glycine in the biosynthesis of thiamine. The variant H244K shows a higher substrate specificity ratio for glycine versus sarcosine and a 5-fold improved specific activity in comparison to the wild-type.

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$ 265.00
$ 89.00
Cat# ABV11810-100
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