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Anti-HIF-1-alpha, Rabbit Monoclonal Antibody

Rabbit Monoclonal Antibody

     
  • IHC - Anti-HIF-1-alpha, Rabbit Monoclonal Antibody ABV11822-50
    Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using anti-HIF-1-alpha monoclonal antibody at 1:1000 dilution.
  • WB - Anti-HIF-1-alpha, Rabbit Monoclonal Antibody ABV11822-50
    Western blot of Jurkat cell lysate, treated or untreated with Cobalt(Ⅱ) chloride(CoCl2), using anti-HIF-1-alpha monoclonal antibody at 1:1000 dilution, showed that HIF-1-alpha (~120kDa) expression was induced by CoCl2 in Jurkat cells.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q16665
Reactivity Human
Host Rabbit
Clonality Monoclonal
Isotype Rabbit IgG
Calculated MW 92670 Da
Additional Information
Gene ID 3091
Positive Control WB: Jurkat cell lystae; IHC: human breast cancer tissue
Application & Usage IHC: 1:500 -1:1000 dilution; WB: 1:1000 - 1:2000 dilution.
Alias Symbol HIF1A
Other Names ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Hypoxia-inducible factor 1-alpha, bHLHe78, Member of PAS protein 1
Appearance Colorless liquid
Formulation In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsAnti-HIF-1-alpha, Rabbit Monoclonal Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HIF1A
Synonyms BHLHE78, MOP1, PASD8
Function Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
Cellular Location Cytoplasm. Nucleus Nucleus speckle {ECO:0000250|UniProtKB:Q61221}. Note=Colocalizes with HIF3A in the nucleus and speckles (By similarity) Cytoplasmic in normoxia, nuclear translocation in response to hypoxia (PubMed:9822602). {ECO:0000250|UniProtKB:Q61221}
Tissue Location Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. A higher level expression seen in pituitary tumors as compared to the pituitary gland
Research Areas
Citations (0)

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Background

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.

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$ 285.00
Cat# ABV11822-50
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