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Anti-Cyclin D1, Rabbit Monoclonal Antibody

Rabbit Monoclonal Antibody

     
  • IHC - Anti-Cyclin D1, Rabbit Monoclonal Antibody ABV11823-50
    Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using anti-Cyclin D1 monoclonal antibody at 1:1000 dilution.
  • WB - Anti-Cyclin D1, Rabbit Monoclonal Antibody ABV11823-50
    Western blot of Hela cell lysates using anti-Cyclin D1 monoclonal antibody at 1:1000 dilution, showed a band of Cyclin D1 (~34kDa) expressed in Hela cells.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P24385
Reactivity Human
Host Rabbit
Clonality Monoclonal
Isotype Rabbit IgG
Calculated MW 33729 Da
Additional Information
Gene ID 595
Positive Control WB: Hela cell lysate; IHC: human breast cancer tissue
Application & Usage IHC: 1:500 -1:1000 dilution; WB: 1:1000 - 1:2000 dilution.
Alias Symbol CCND1
Other Names G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, PRAD1
Appearance Colorless liquid
Formulation In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsAnti-Cyclin D1, Rabbit Monoclonal Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCND1
Synonyms BCL1, PRAD1
Function Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
Cellular Location Nucleus. Cytoplasm. Membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members
Research Areas
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Background

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.

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$ 285.00
Cat# ABV11823-50
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