|Application ||WB, IHC|
|Positive Control||WB: lysate from cell line expressing V600; IHC: melanoma tissue; ELISA: 293T cells overxpressing BRAF V600|
|Application & Usage||IHC, ICC: 2 – 10 µg/mL; ELISA: 0.5 – 2 µg/mL; WB: 0.5 – 2 µg/mL.|
|Formulation||In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide|
|Reconstitution & Storage||-20 °C|
|Precautions||Anti-BRAF V600E Rabbit Monoclonal Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Serine/threonine-protein kinase B-raf (BRAF) is a member of the Raf family. BRAF mutations are frequent in benign and malignant human tumors. BRAF V600E mutation accounts for the vast majority of BRAF alterations and the mutation induces a conformational change of the activation segment leading to a constitutive kinase activity of BRAF and consecutive phosphorylation of downstream targets. BRAF V600E mutation have been detected in melanoma, papillary thyroid carcinoma, pleomorphic xanthoastrocytomas, Langerhans cell histiocytosis, borderline ovarian cancer, ganglioglioma, colorectal carcinoma, and pilocytic astrocytoma.
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