Anti- His-Tag Rabbit Monoclonal Antibody
Rabbit Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, FC, ICC, IP |
---|---|
Host | Rabbit |
Clonality | Monoclonal |
Isotype | Rabbit IgG |
Positive Control | WB: 293T cell lysate; IP: 293T cells; IHC: HepG2 cells; ICC: 293T cells |
---|---|
Application & Usage | Western Blot: 0.1 ug/mL – 1 ug/mL;Immunoprecipitation: 0.5 ug/mL – 2 ug/mL;Immunocytochemistry 0.5 ug/mL – 2 ug/mL;Flow Cytometry: 0.5 ug/mL – 2 ug/mL;Immunohistochemistry; 0.1 ug/mL-1 ug/mL. |
Alias Symbol | His |
Other Names | His-Tag |
Appearance | Colorless liquid |
Formulation | In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Anti- His-Tag Rabbit Monoclonal Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
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