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Anti-Histone H2AX Rabbit Monoclonal Antibody
Rabbit Monoclonal Antibody
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC, E |
|---|---|
| Primary Accession | P16104 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 15145 Da |
| Gene ID | 3014 |
|---|---|
| Positive Control | WB: A375, HEK293, HeLa and SK-MEL-2; ICC: HeLa cells |
| Application & Usage | Western Blot: 0.5 ug/mL – 2 ug/mL; ICC: 1 ug/mL - 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.2 ug/mL – 1 ug/mL. |
| Alias Symbol | H2AFX |
| Other Names | H3F3A, H3.3A, H3F3, H3F3B, H3.3B |
| Appearance | Colorless liquid |
| Formulation | In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | Anti-Histone H2AX Rabbit Monoclonal Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | H2AX (HGNC:4739) |
|---|---|
| Function | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. |
| Cellular Location | Nucleus. Chromosome |
Provided below are standard protocols that you may find useful for product applications.
Background
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
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