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Anti-Histone H2B Rabbit Monoclonal Antibody

Rabbit Monoclonal Antibody

     
  • ICC - Anti-Histone H2B Rabbit Monoclonal Antibody ABV11838-25
    Immunocytochemistry of Hela cells using Anti-Histone H2B Rabbit mAb (red). Actin filaments have been labeled with fluorescein phalloidin(green).
    detail
  • WB - Anti-Histone H2B Rabbit Monoclonal Antibody ABV11838-25
    Western blot of recombinant Histone H2A, H2B, the whole cell lysates of Hela, A375, SK-MEL-2, A431 and K562, using anti-Histone H2B rabbit mAb at 0.2ug/ml.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC, E
Primary Accession P06899
Reactivity Human
Host Rabbit
Clonality Monoclonal
Isotype Rabbit IgG
Calculated MW 13904 Da
Additional Information
Gene ID 8970
Positive Control WB: HeLa, A375, SK-MEL-2, A431, and K562 cell lyastes; ICC: HeLa cells
Application & Usage Western Blot: 0.1 ug/mL – 1 ug/mL; ICC: 0.5 µg/mL - 1 µg/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.2 ug/mL – 1 ug/mL.
Alias Symbol HIST1H2BJ
Other Names Histone H2B type 1-J, Histone H2B.1, Histone H2B.r, H2B/r
Appearance Colorless liquid
Formulation In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsAnti-Histone H2B Rabbit Monoclonal Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name H2BC11 (HGNC:4761)
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Research Areas
Citations (0)
citation

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Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium and to the bactericidal activity of amniotic fluid.

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Discontinued
Cat# ABV11838-25
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