|Application ||WB, IHC, IF, ICC|
|Calculated MW||115281 Da|
|Positive Control||WB: HEK293 cell lysates; IHC: human breast cancer tissue; IFC: HEK293 cells|
|Application & Usage||WB; 1:500 – 1:2000, IHC; 1:50 – 1:200, IF/IC; 1:50 – 1:100|
|Other Names||MBS, MYPT1, Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit, Myosin phosphatase|
|Formulation||In 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol and 0.01% sodium azide|
|Reconstitution & Storage||-20 °C|
|Precautions||Phospho-MYPT1 (Thr696) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity.|
|Cellular Location||Cytoplasm. Note=Along actomyosin filaments and stress fibers|
|Tissue Location||Expressed in striated muscles, specifically in type 2a fibers (at protein level).|
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Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.
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