|Reactivity||Human, Mouse, Rat|
|Calculated MW||50141 Da|
|Positive Control||WB: H9C2 PMA-treated (A) whole cell lysates|
|Application & Usage||WB; 1:500 – 1:2000|
|Other Names||AcK146, EEF1A1, EEF1A, EF1A, LENG7, Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu; Eukaryotic elongation factor 1 A-1, eEF1A-1, Leukocyte receptor cluster member 7, EEF1A2, EEF1AL, STN, Elongation factor 1-alpha 2, EF-1-alpha-2, Eukaryotic elongation factor 1 A-2, eEF1A-2, Statin-S1, EEF1A1P5, EEF1AL3, Putative elongation factor 1-alpha-like 3, EF-1-alpha-like 3, Eukaryotic elongation factor 1 A-like 3, eEF1A-like 3, Eukaryotic translation elongation factor 1 alpha-1 pseudogene 5|
|Handling||The antibody solution should be gently mixed before use|
|Reconstitution & Storage||-20°C|
|Precautions||Anti-EEF1A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||EEF1A, EF1A, LENG7|
|Function||This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.|
|Cellular Location||Cytoplasm. Nucleus. Nucleus, nucleolus. Cell membrane. Note=Colocalizes with DLC1 at actin-rich regions in the cell periphery (PubMed:19158340) Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens (PubMed:8650580) Localization at the cell membrane depends on EEF1A1 phosphorylation status and the presence of PPP1R16B (PubMed:26497934).|
|Tissue Location||Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.