|Reactivity||Human, Mouse, Rat|
|Calculated MW||117904 Da|
|Positive Control||WB: PC3, mouse barin, rat brain lysates|
|Application & Usage||WB; 1:500 – 1:2000|
|Other Names||s GLI; Zinc finger protein GLI1; Glioma-associated oncogene; Oncogene GLI|
|Handling||The antibody solution should be gently mixed before use|
|Reconstitution & Storage||-20°C|
|Precautions||Anti-GLI1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a transcriptional activator (PubMed:19706761, PubMed:10806483, PubMed:19878745, PubMed:24311597, PubMed:24217340). Binds to the DNA consensus sequence 5'- GACCACCCA-3' (PubMed:2105456, PubMed:8378770, PubMed:24217340). May regulate the transcription of specific genes during normal development (PubMed:19706761). May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed:19706761). Plays a role in cell proliferation and differentiation via its role in SHH signaling (Probable).|
|Cellular Location||Cytoplasm. Nucleus. Note=Tethered in the cytoplasm by binding to SUFU (PubMed:10806483). Activation and translocation to the nucleus is promoted by interaction with STK36 (PubMed:10806483). Phosphorylation by ULK3 may promote nuclear localization (PubMed:19878745). Translocation to the nucleus is promoted by interaction with ZIC1 (PubMed:11238441)|
|Tissue Location||Detected in testis (at protein level) (PubMed:2105456). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract (PubMed:19878745). Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues (PubMed:19706761)|
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