|Application ||WB, IHC, E|
|Calculated MW||30382 Da|
|Positive Control||WB: COLO205 cell lysate|
|Application & Usage||WB 1:500-1:2000; IHC 1:100-1:300; E 1:40000|
|Other Names||PRKAB1; AMPK; 5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb|
|Handling||The antibody solution should be gently mixed before use|
|Reconstitution & Storage||-20°C|
|Precautions||Phospho-AMPKβ1 (Ser182) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.